MEM α, Nucleosides, No Phenol Red

Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells

Overview
Brand: Gibco

Manufacturer Part Number: 41061029

MEM ALPHA

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.58000kg



Requires: Supplementation

Product Code. 11534476

Quantity Price
1 £ 39.86 / 500mL
EU Stock 74
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Format Liquid
Formulation 2'Deoxyguanosine, 2Deoxyadenosine, 2Deoxycytidine HCl, Adenosine, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cytidine, D-Calcium pantothenate, Folic Acid, Glycine, Guanosine, L-Alanine, L-Arginine, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Thymidine, Uridine, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Quantity 500mL
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives Sodium Pyruvate; Low Glucose; L-Glutamine; No Phenol Red

MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.

  • Modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid
  • Available without nucleosides for use as selection medium for DG44 and other DHFR negative cells
  • Modified as follows: with ribonucleosides, deoxyribonucleosides, L-glutamine; without phenol red
  • Made with Earle's salts and contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • is product is made with Earle's salts
  • Suatainable packaging

Cell Culture, Mammalian Cell Culture

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