MEM, NEAA, Powder

Minimum Essential Medium is commonly used of all cell culture media

Brand: Gibco

Manufacturer Part Number: 41500018


UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.09000kg

Requires: Supplementation

Product Code. 11534486

Quantity Price
1 £18.16 / 10 L
EU Stock 53
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Format Powder
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
Packaging Sustainable packaging
Quantity 10L
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
With Additives No Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.

  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Modified as follows: with NEAA, L-glutamine, phenol red; without HEPES, sodium bicarbonate
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation

Cell Culture, Mammalian Cell Culture

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