Medium 199, Powder

Originally developed for nutritional studies of chick embryo fibroblasts

Brand: Gibco

Manufacturer Part Number: 31100019

1 LT MEDIUM 199, POWDER WITH EARLE'S SALTS, L-GLUTamine without sodium hydrogen carbonate Store at

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.49000kg

Product Code. 11534426

Quantity Price
1 £57.25 / 50 L
EU Stock 1
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Format Powder
Formulation Adenine sulfate, Adenosine 5-phosphate, Adenosine 5-triphosphate, Alpha-tocopherol Phosphate, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Cholesterol, Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Deoxyribose, Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Guanine hydrochloride, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Menadione (Vitamin K3), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Ribose, Sodium Acetate, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Thymine, Tween 80, Uracil, Vitamin A (acetate), Vitamin D2 (Calciferol), Xanthine-Na, i-Inositol
Quantity 50L
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
With Additives No Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

  • It is modified as follows: with L-glutamine and phenol red; without HEPES or sodium bicarbonate
  • Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Contains no proteins, lipids, or growth factors
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at time of preparation

Cell Culture, Mammalian Cell Culture

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