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Gibco™ Medium 199, Earle's Salts, powder

Product Code. 11534426
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Quantity:
50 L
Unit Size:
50 L
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Product Code. 11534426

Brand: Gibco™ 31100019

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Using Medium 199

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Información de órdenes

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Especificaciones

Cell Line Rat epithelial
Cell Type Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Form Powder
With Additives Low Glucose, Phenol Red
Without Additives No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 50 L
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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