Gibco™ Human CCL2 (MCP-1) Recombinant Protein

Product Code. 10785593

Brand: Gibco™ PHC1011

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to 0.1-0.5 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycle.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Specifications

• Accession Number: P13500
• For Use With (Application): Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 6347
• Molecular Weight (g/mol): 8 kDa
• Name: Human CCL2 (MCP-1)
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: 4°C
• Regulatory Status: RUO
• Endotoxin Concentration: ≤0.1 ng/μg
• Gene Alias: Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; Mcp1; MCP-1; MCP1A; MCP-1A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; SCYA2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF
• Common Name: CCL2 (MCP-1)
• Gene Symbol: Ccl2
• Biological Activity: ED50 ≤ 10 ng/mL; determined by the dose-dependent chemotaxis of human THP-1 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: 4°C
• Classification: Carrier-Free
• Activity: ED50 ≤ 10 ng/mL; determined by the dose-dependent chemotaxis of human THP-1 cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Chemokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: MCP⁄MCAF Chemokines
• Research Category: Signal Transduction, Inflammation, Bone Research
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE