Gibco™ Mouse IL-4 Recombinant Protein

Product Code.	Quantity	unitSize
10150913	10 μg	Each
10028792	25 μg	Each

Product Code. 10150913 Supplier Gibco™ Supplier No. PMC0045

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized mIL-4 should be reconstituted in sterile, 10 mM HAc not less than 100 μg/mL, which can then be further diluted to other aqueous solutions. For long term storage, it is recommended to add a carrier protein, such as 0.1% HSA or BSA. Storage: This lyophilized preparation is stable at room temperature for 3 weeks, but should be stored desiccated below -18°C. Upon reconstitution, the preparation is stable at 4°C for up to 1 week, but should be stored below -18°C for long term storage. Avoid repeated freeze/thaw cycles.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Accession Number: P07750
• For Use With (Application): Bioactivity
• Formulation: PBS with no preservative; pH 7.4
• Gene ID (Entrez): 16189
• Molecular Weight (g/mol): 13.5 kDa
• Name: Mouse IL-4
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Common Name: IL-4
• Gene Symbol: Il4
• Biological Activity: ED50 = 2 ng/mL; determined by the dose-dependent proliferation of mouse HT-2 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Sequence: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
• Content And Storage: -20°C
• Activity: ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of mouse HT-2 cells.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Immunology, Stem Cell Research, Inflammation
• Product Line: Gibco
• Purity or Quality Grade: >96% by SDS-PAGE and RP-HPLC