Gibco™ Human IL-12 Recombinant Protein

Product Code. 10023103

Brand: Gibco™ PHC1124

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in phosphate buffered saline, pH 7.2-pH 7.4, to a concentration of ≥0.2 mg/mL. Further dilutions should be made in sterile medium or buffered solution with a carrier protein. Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at ≤-20°C. Avoid repeated freeze/thaw cycles.

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

Specifications

• Accession Number: P29459, P29460
• For Use With (Application): Bioactivity
• Formulation: PBS with no preservative; pH 7.4
• Gene ID (Entrez): 3592, 3593
• Molecular Weight (g/mol): 75 kDa
• Name: Human IL-12
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: 20C2; CLMF; CLMF p35; CLMF p40; CLMF2; cytokine; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; Il12; IL-12; Il-12 p35; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il-12; cytokine; IL12A; IL-12A; IL12A/B; IL12AB; IL12B; Il-12b; IL12P35; IL-12p35; Il12p40; Il-12p40; IL35 subunit; ILN; IMD28; IMD29; Interleukin; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 p40 subunit precursor; interleukin 12 polypeptide 35; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 12p35 subunit precursor; interleukin-12 alpha chain; interleukin-12 alpha p40 subunit; interleukin-12 beta chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 p35 subunit precursor; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 p40 subunit precursor; Interleukin-12 p70; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-23 p40 subunit; Ll12a; MGC151228; MGC151232; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35; p40
• Common Name: IL-12 p70
• Gene Symbol: IL12A, Il12b
• Biological Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent IFN-gamma production of NK cells stimulated with IL-18.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human IL-12 recombinant protein is a heterodimer composed of disulfide-linked p35 and p40 subunits
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent IFN-gamma production of NK cells stimulated with IL-18.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: CHO cells
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Immunology, Stem Cell Research, Oncology, Signal Transduction
• Product Line: Gibco
• Purity or Quality Grade: ≥95.5% by SDS-PAGE and HPLC