missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Buffers and Standards
  4. Life Science Buffers
  5. Gibco™ HBSS, calcium, magnesium, no phenol red 

Gibco™ HBSS, calcium, magnesium, no phenol red
GREENER_CHOICE

Product Code. 15266355
Change view
Click to view available options
Quantity:
500 mL
Unit Size:
500mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
15266355 500 mL 500mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 15266355 Supplier Gibco™ Supplier No. 14025092

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.

This HBSS is modified as follows:

With: Calcium, Magnesium, Glucose

Without: Phenol Red, Sodium Pyruvate

The complete formulation is available.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Hanks' Balanced Salt Solution (HBSS)
Color Clear
Concentration 1 X
For Use With (Application) Mammalian Cell Culture
pH 6.7 - 7.8
Physical Form Liquid
Product Line Gibco
Quantity 500 mL
Solution Type Hank's Balanced Salt
Sterility Sterile-filtered
Includes No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Osmolality 270 - 305 mOsm/kg
Show More Show Less

Frequently Asked Questions (FAQs)

What is the pH of HBSS, calcium, magnesium, no phenol red at room temperature.

At room temperature, HBSS, calcium, magnesium, no phenol red has a pH of 6.7 - 7.8.
Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.
Do I need to add additional HEPES to Hank's Balanced Salt Solution (HBSS) (Cat. No. 14025xxx)?

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).
I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.
Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.
Why do you offer HBSS with and without phenol red?

Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.
Can I use HBSS (with calcium and magnesium) to wash my cells before dissociation?

No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.
Is a precipitate in HBSS (with calcium and magnesium) normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.
Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.