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Gibco™ Grace's Insect Medium, unsupplemented, powder
Description
Requires
Requires supplementation with 10% Fetal Bovine Serum (FBS), 3.33g/L lactalbumin hydrolysate and 3.33g/L yeastolate before use as a growth medium as well as ambient CO2 in order to maintain physiological pH. Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation.
Widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. It is modified as follows: with L-glutamine, without Lactalbumin hydrolysate, Yeastolate, Sodium bicarbonate. It does not contain lactalbumin hydrolysate and yeastolate commonly found in the supplemented version
Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture
Order Info
Shipping Condition: Room temperature
Compliance
Manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Specifications
| Cell Type | Insect Cell |
| Form | Powder |
| Product Type | Grace's Insect Medium |
| With Additives | Glutamine |
| Without Additives | No HEPES, No Phenol Red, No Sodium Pyruvate, No Lactalbumin Hydrolysate, No Yeastolate, No Sodium Bicarbonate |
| Product Line | Gibco™ |
| Quantity | 10 x 1 L |
| Shipping Condition | Room Temperature |
| Content And Storage | Storage conditions: 2-8°C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
For Research Use Only. Not for use in diagnostic procedures.
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