Gibco™ DPBS, no calcium, no magnesium

Product Code.	Quantity	Packaging	unitSize
12559069	10 x 500 mL	Bottle	Pack of 10
15326239	500 mL	Bottle	500mL
10444402	1000 mL	Bottle	1 L
11544556	5 L	Bag	5 L
11554556	10 L	Bag	10 L
11340972	20 L	Bag	Each

Product Code. 12559069 Supplier Gibco™ Supplier No. 14190250

Description

Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

This DPBS is modified as follows:

Without: Calcium, Magnesium, Phenol Red

Order Info

Shipping Condition: Room Temperature

Specifications

• Chemical Name or Material: DPBS
• Color: Clear
• Concentration: 1 X
• For Use With (Application): Mammalian Cell Culture
• pH: 7.0 to 7.3
• Physical Form: Liquid
• Product Line: Gibco
• Quantity: 10 x 500 mL
• Solution Type: Dulbecco's Phosphate Buffered Saline
• Packaging: Bottle
• Sterility: Sterile-filtered
• Includes: No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Osmolality: 270 - 300 mOsm/kg

Frequently Asked Questions (FAQs)

What is the difference between DPBS, no calcium, no magnesium, 10 x 500 mL, Cat. Nos. 14190250 and 1490169?

DPBS, no calcium, no magnesium, 10x 500 mL (Cat. Nos. 14190250 and 14190169) have the same formulation. The only difference is in the manufacturing site.

The DPBS, no calcium, no magnesium (Cat. no. 14190169) is manufactured in our Inchinnan, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. DPBS, no calcium, no magnesium (Cat. No. 14190250) is manufactured in Grand Island, US, and is recommended for our customers in North America, Latin America, and the Asia/Pacific region.

The complete formulation can be found here:

14190 - DPBS, no calcium, no magnesium

What is the shelf life of DPBS after it has been opened?

We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.

What is the shelf life of DPBS (Cat. No. 14190136)?

DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?

Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.

Which cell lines can I culture in Diploid Growth Serum-Reduced Medium (SRM)?

Diploid Growth Serum-Reduced Medium has been evaluated with fibroblast cell lines, including MRC-5, WI-38, IMR-90, BS-2, and CEF cells.

How long can I use Diploid Growth Serum-Reduced Medium (SRM) supplemented with Diploid Growth Supplement?

Once supplemented, the complete Diploid Growth Serum-Reduced Medium must be used within 4 weeks.

Can I freeze or thaw my cells in Diploid Growth Serum-Reduced Medium (SRM)?

Most diploid cell lines can be frozen and thawed in Diploid Growth Serum-Reduced Medium. Consult the Diploid Growth Serum Reduced Medium User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019015_DiploidGrowthSerumReducedMedium_UG.pdf) for additional information.

How do I adapt my cells to Diploid Growth Serum-Reduced Medium (SRM)?

Cells growing in medium with serum can be transferred directly to Diploid Growth Serum-Reduced Medium without adaptation. Continue to monitor and passage cells for 2-3 passages until consistent growth is achieved. Cultures may benefit from supplementation with 1-2% serum.

Can I get a specific value for the conductivity for your DPBS products?

Sorry, we do not test the conductivity of our DPBS products and hence cannot provide a value or range.

Are your DPBS products DNase/RNase-free? Can I use them for RNA work?

Our DPBS products are for cell culture use and have not been tested for DNase/RNase activity. We do not recommend using these products for RNA work.

What is the specific gravity of DPBS (without calcium and magnesium)?

The specific gravity of this product is approximately 1.006 kg/L.

What is the overall molarity of DPBS (without calcium and magnesium)?

The overall molarity of this solution is 19.66. This is calculated by taking the sum of the dry weights and dividing it by the sum of the formula weight.

Is a precipitate in DPBS (without calcium and magnesium) normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.