Gibco™ DMEM, Powder, Low Glucose, Pyruvate, No Sodium Bicarbonate Buffer

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, Low Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications.

Overview
Brand: Gibco™

Manufacturer Part Number: 31600091

DMEMpowder (low glucose), for 50 L

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.49000kg



Requires: Supplementation
Disclaimers: In vitro diagnostic; Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

Product Code. 10144602

Quantity Price
1 £ 73.0 / Each
Estimated Shipment date
from Supplier 15-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Mammalian cell culture
Format Powder
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, i-Inositol
Product Type DMEM media
Quantity 50L
Shelf Life 36 months from date of manufacture
Storage Requirements Storage: 2-8° C
With Additives Low Glucose, L-glutamine, Sodium Pyruvate, Phenol Red
Without Additives Sodium Bicarbonate, HEPES

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info: Shipping Condition: Room Temperature

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