DMEM, Powder, Low Glucose, Pyruvate, No Sodium Bicarbonate Buffer

Dulbecco's Modified Eagle Medium is widely used basal medium for supporting growth of many different mammalian cells

Brand: Gibco

Manufacturer Part Number: 31600091

DMEMpowder (low glucose), for 50 L

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.49000kg

Requires: Supplementation

Product Code. 10144602

Quantity Price
1 £69.00 / Each
Estimated Shipment date
from Supplier 26-10-2016
Add to basket

For Research Use Only. All usage must comply with product instructions.

Description and Specification


Format Powder
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, i-Inositol
Quantity 50L
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
With Additives Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

  • Unique from other media as it contains four times the concentration of amino acids and vitamins than original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • It is modified as follows: with low glucose, L-glutamine, sodium pyruvate, phenol red; without sodium bicarbonate, HEPES
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at time of preparation


Cell Culture, Mammalian Cell Culture

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.