Gibco™ DMEM, powder, high glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 12800017

Product Code. 11588826

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

• Content And Storage: Storage conditions: 2-8° C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Form: Powder
• Serum Level: Standard Serum Supplementation
• With Additives: High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 1 L

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Classification: Animal Origin-free
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Bicarbonate
• Product Line: Gibco™
• Shipping Condition: Room Temperature

In vitro diagnostic