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Gibco™ DMEM, high glucose, no glutamine, no methionine, no cystine

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 21013024

18.11 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.58000kg

Product Code. 11570546

  • £27.17 / 500mL
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For Research Use Only. All usage must comply with product instructions.
 

Requires : Supplementation

Description

Description

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, Phenol Red
Without: L-glutamine, Sodium Pyruvate, HEPES, L-methionine, L-cystine

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco DMEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

No Cystine, No Methionine
HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Phenol Red
Sustainable packaging
Gibco™
Room Temperature
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)
Sterile-filtered
No Glutamine, No HEPES, No Sodium Pyruvate, No Methionine, No Cystine
cGMP-compliant under the ISO 13485 standard
500 mL
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In vitro diagnostic. For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.