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Gibco™ DMEM, high glucose, no glutamine

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 11960044

13.78 GBP valid until 2024-03-31
Use promo code "21883" to get your promotional price.



Code : 50

Additional Details : Weight : 0.50000kg

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Product Code. 11500416

  • £20.17 / 500mL
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, Phenol Red
Without: L-glutamine, Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Specifications

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)
Sterile-filtered
No Glutamine, No HEPES, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
500 mL
HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Phenol Red
Sustainable packaging
Gibco™
Room Temperature
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.