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Gibco™ DMEM, high glucose, NEAA, no glutamine
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer is used in human ex vivo tissue and cell culture processing applications.
Brand: Gibco™ 10938025
Code : 50
Additional Details : Weight : 0.50000kg
Description
- Animal-origin free
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco DMEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Specifications
| Animal Origin-Free | |
| CO2 | |
| 310 - 360 mOsm/kg | |
| Standard Serum Supplementation | |
| Sodium Bicarbonate | |
| 500 ml | |
| High Glucose | |
| Phenol Red | |
| 7.0 - 7.2 |
| Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture. |
|
| No HEPES | |
| Gibco™ | |
| Room Temperature | |
| Serum | |
| Liquid | |
| No Glutamine | |
| No Sodium Pyruvate | |
| 1 X |
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.