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Gibco™ DMEM, high glucose, NEAA, no glutamine

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer is used in human ex vivo tissue and cell culture processing applications.

Brand:  Gibco™ 10938025

Code : 50

Additional Details : Weight : 0.50000kg

Product Code. 11590366

  • £12.25 / 500mL

In stock, ready to ship: 552

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For Research Use Only. All usage must comply with product instructions.



  • Animal-origin free
  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature


Gibco DMEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.



Animal Origin-Free
310 - 360 mOsm/kg
Standard Serum Supplementation
Sodium Bicarbonate
500 ml
High Glucose
Phenol Red
7.0 - 7.2
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Room Temperature
No Glutamine
No Sodium Pyruvate
1 X

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.