Gibco™ DMEM, high glucose, HEPES

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, HEPES is used in cell culture processing applications.

Brand:  Gibco™ 12430054

Product Code. 10174552

Description

• Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Green Features: Sustainable packaging
• Product Line: Gibco™
• Serum Supplementation: Standard Serum Supplementation
• Volume (Metric): 500 ml
• Form: Liquid
• Glutamine: L-Glutamine
• Sodium Pyruvate Additive: No Sodium Pyruvate

• Glucose Concentration: 4500 mg/L
• HEPES Buffer: HEPES
• Quantity: 500mL
• Shipping Condition: Room Temperature
• For Use With (Application): Mammalian cell culture
• Glucose: High Glucose
• Phenol Red Indicator: Phenol Red

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco™ DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.