DMEM, High Glucose, GlutaMAX™ Supplement, Pyruvate

Dulbecco's Modified Eagle Medium is a widely used basal medium for supporting growth of many different mammalian cells

Brand: Gibco

Manufacturer Part Number: 10569077


UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 11.27000kg

Requires: Supplementation

Product Code. 11370872

Quantity Price
1 £255.00 / Each
Estimated Shipment date
from Supplier 25-10-2016
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Format Liquid
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Alanyl-Glutamine, L-Arginine hydrochloride, L-Cystine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, i-Inositol
Packaging Sustainable packaging
Quantity 10L
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives Sodium Pyruvate; High Glucose; GlutaMAX™-I; Phenol Red

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

  • Modified as follows: with high glucose, sodium pyruvate, GlutaMAX™ and phenol red; without HEPES
  • Contains four times concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
  • GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7g/L)
  • Requires 5-10% CO2 environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

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