Gibco™ DMEM, High Glucose

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.

Brand: Gibco™

Manufacturer Part Number: 11965167


UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 5.62000kg

Requires: Supplementation
Disclaimers: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.

Product Code. 11330882

Quantity Price
1 £140.00 / Each
Estimated Shipment date
from Supplier 01-11-2016
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


For Use With (Application) Antibody and cell culture production, Mammalian cell culture
Format Liquid
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
Packaging Sustainable packaging
Product Type DMEM media
Quantity 5L
Shelf Life 12 months from the date of manufacture
Storage Requirements Storage: 2-8° C. Protect from light
With Additives High Glucose, L-glutamine, Phenol Red
Without Additives Sodium Pyruvate, HEPES

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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