Gibco™ DMEM/F-12, GlutaMAX™ Supplement

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, GlutaMAX™ Supplement is used in cell culture processing applications.

Overview
£15.53 valid until 30/04/2017
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Brand: Gibco™

Manufacturer Part Number: 10565018

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 1.05000kg



Requires: Supplementation

Product Code. 11559726

Quantity Price
1 £ 19.42 / 500mL
EU Stock 96
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Description and Specification

Specification

With Additives GlutaMAX™ Supplement, Phenol Red
Storage Requirements Storage: 2-8° C. Protect from light
For Use With (Application) Mammalian cell culture
Product Type DMEM/F-12 media
Shelf Life 12 months from the date of manufacture
Without Additives HEPES
Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Alanyl-L-Glutamine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Format Liquid
Packaging Sustainable packaging
Quantity 500mL

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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