Gibco™ DMEM/F-12, GlutaMAX™ supplement

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Product Code	Brand	Quantity
11559726	Gibco™ 10565018	500 mL

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:
With: GlutaMAX, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No HEPES

• Concentration: 1 X
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
• With Additives: High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.