Advanced MEM, HEPES

Advanced Minimum Essential medium is widely used basal medium that allows culture of mammalian cells with reduced Fetal Bovine Serum supplementation

Overview
Brand: Gibco

Manufacturer Part Number: 12492021

5000ML X10 Advanced MEM in plastic bottle store at

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 6.72000kg



Requires: Supplementation

Product Code. 11540436

Quantity Price
1 £ 130.0 / 5000mL
EU Stock 15
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Format Liquid
Formulation AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, Folic Acid, Glycine, Human Transferrin (Holo), Insulin Recombinant Full Chain, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-H2O), Thiamine hydrochloride, i-Inositol
Packaging Sustainable packaging
Quantity 10 x 500mL
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives Sodium Pyruvate; Low Glucose; No Glutamine; Phenol Red

Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2. It is modified as follows: with glucose, non essential amino acids, sodium pyruvate, phenol red; without L-glutamine, HEPES.

  • Unique from other media due to addition of following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
  • Requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement
  • Many cell lines do not require adaptation to this media
  • FBS concentration must be optimized for each cell line to obtain maximum serum reduction
  • Uses sodium bicarbonate buffer system (3.7g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture