Gibco™ Advanced DMEM

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEM is used in cell culture processing applications.

Overview
Brand: Gibco™

Manufacturer Part Number: 12491015

ADVANCED D-MEM

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.58000kg



Requires: Supplementation

Product Code. 11510436

Quantity Price
1 £ 15.43 / 500mL
EU Stock 752
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Mammalian cell culture
Format Liquid
Formulation AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric Sulfate, D-Calcium pantothenate, D-Glucose (Dextrose), Ethanolamine, Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Human Transferrin (Holo), Insulin Recombinant Full Chain, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Manganous Chloride, Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-H2O), Sodium Pyruvate, Sodium Selenite, Thiamine hydrochloride, i-Inositol
Packaging Sustainable packaging
Product Type DMEM media
Quantity 500mL
Shelf Life 12 months from date of manufacture
Storage Requirements Storage: 2-8° C. Protect from light
With Additives High Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red
Without Additives L-glutamine

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12