Thermo Scientific™ GFP-4E-BP1

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).

Brand:  Thermo Scientific™ PV4759

Product Code. 10042813

Description

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).

For use in combination with the LanthaScreen™ Tb-anti-4E-BP1 [pThr46] Antibody (Cat. No. PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.

Specifications

• Assay Entry: Biochemical terbium phospho-specific activity
• Detection Method: Fluorescence
• For Use With (Application): Pharma and biopharma, protein assays and analysis, target and lead identification and validation
• Substrate: 4E-BP1
• Substrate Type: Kinase Substrate
• Item Description: GFP-4E-BP1, 20nmol, 20.02μM Concentration, 45600Da Molecular Weight, Fluorescent Detection Method, GFP (EmGFP Label, Serine/Threonine Kinases Target Enzyme, Used For Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and
• Molecular Weight (g/mol): 45,600 Da
• Product Type: GFP-4E-BP1
• Storage Requirements: –80°C
• Validated Kinases: FRAP1 (mTOR), FRAP1 (mTOR)

• Content And Storage: GFP-4E-BP1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 2 mM DTT
  
  Store at -80°C.
• Form: Liquid
• Quantity: 20 nmol
• Substrate Properties: Protein-Based Substrate
• Target Enzyme: Serine/Threonine Kinases
• Label or Dye: GFP (EmGFP)
• Packaging: 1 Each
• Shipping Condition: Dry Ice
• Target Entry: mTOR, FRAP1

For Research Use Only. Not for use in diagnostic procedures.