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Macherey-Nagel™ Lysis Buffer Set PL2/PL3

Product Code. 12830911
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Product Code. 12830911 Supplier Macherey-Nagel™ Supplier No. 740919

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Description

Lysis Buffer PL2 and Precipitation Buffer PL3 to be used with NucleoSpin Plant II kits.

Includes two optimised, alternative lysis buffers based on the established CTAB and SDS lysis methods. As plants are very heterogenous and contain many different metabolites such as polyphenols, polysaccharides, or acidic components, NucleoSpin™ Plant II offers two different lysis proceudres for optimal processing of various samples. The silica membrane in the NucleoSpin™ Plant II columns is optimised to improve DNA binding. NucleoSpin™ filters ar included for convenient clarification of lysates. NucleoSpin™ Plant II allows processing of up to 100 mg (wet weight) or 20mg (dry weight) starting material. Depending on the individual sample typical yields are in the range from 1μg to 30μg DNA. The eluted DNA is ready to use for subsequent reactions such as PCR*, restriction analysis and others. Kit components: NucleoSpin™ Plant II columns, filters, collecting tubes 2mL, buffers, RNase A.
  • Silica membrane technology
  • Yield: 1μg to 30μg
  • Sample size: ≤100 mg wet weight ≤20mg dry weight
  • Elution volume: 100μL
  • Binding capacity: 50μg
  • Preparation time: 30 min./prep
  • NucleoSpin filters included for clarification of lysates
  • RNase A included
  • Format: mini spin column
  • Two alternative lysis buffers included for processing of various samples (PL1 uses CTAB lysis method, PL2 uses SDS lysis method) For isolation of genomic DNA from plant and fungal material.

Specifications

Chemical Name or Material Lysis Buffer
For Use With (Application) Lysis Buffer, with 100mL Buffer PL2 and 25mL Buffer PL3, For Genomic DNA Isolation
Quantity 1 Set
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