missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10063533

Thermo Scientific™ GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells

Product Code. 10063533
Change view
Click to view available options
Quantity:
2 x 106 Cells
Unit Size:
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10063533 2 x 106 Cells Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10063533 Supplier Thermo Scientific™ Supplier No. K1695

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Antagonists none
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage GeneBLAzer™ Nuclear Receptor Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use., Store in liquid nitrogen immediately upon receipt, or thaw for immediate use
Detection Method Fluorescence, Fluorescent
Description Primary Agonist: 9-cis-retinoic acid
For Use With (Application) Nuclear Receptors
Druggable Target Nuclear Receptors
No. of Cells 2 x 106
Primary Agonist 9-cis-retinoic acid
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Product Type HEK 293T Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry RAR beta, NR1B2, RARB
Type Dividing Cells
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.