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Thermo Scientific™ GeneBLAzer™ PR-UAS-bla HEK 293T Cells

Product Code. 10003443
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10003443 2 x 106 Cells, 2 x 106 cells Each
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Product Code. 10003443 Supplier Thermo Scientific™ Supplier No. K1693

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Description

PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of R5020. In addition, PR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists GW 3965, 24 (S), 25-Epoxycholesterol, 22 (R) OH-Cholesterol, 24 (S) OH-Cholesterol
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage PR-UAS-bla HEK 293T cells (K1103)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen), -80°C
Detection Method Fluorescence, Fluorescent
Description GeneBLAzer™ PR-UAS-bla HEK 293T Cells, 2 x 106 cells, Liquid, HEK 293T Cell Line, Human Species, Dividing Cells State, Fluorescent Detection Method, Nuclear Receptors Target, BLA (Beta-Lactamase) Reporter, Epithelial Cell Phenotype, Adherent Growth Properties
For Use With (Application) Pharma and biopharma, target and lead identification and validation
Druggable Target Nuclear Receptors
No. of Cells 2 x 106
Primary Agonist TO901317
Product Line GeneBLAzer
Quantity 2 x 106 Cells, 2 x 106 cells
Product Type HEK 293T Cells
Sample Type 293T (HEK)
Reporter Gene BLA (Beta-Lactamase)
Target Entry PGR, NR3C3, PR
Type Cell Line
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For Research Use Only. Not for use in diagnostic procedures.

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