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Thermo Scientific™ GeneBLAzer™ PPARγ-UAS-bla HEK 293H Cells

Product Code. 10175173
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10175173 2 x 106 Cells Each
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Product Code. 10175173 Supplier Thermo Scientific™ Supplier No. K1701

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Description

PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line.

PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line. GeneBLAzer™UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC50 concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage PPAR gamma-UAS-bla 293H cells (K1094)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen), Liquid Nitrogen
Detection Method Fluorescence, Fluorescent
For Use With (Application) Nuclear Receptors
Species Human
Druggable Target Nuclear Receptors
Primary Agonist Calcitriol
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Product Type HEK 293H Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry PPARG, PPAR gamma, NR1C3
Type Dividing Cells
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For Research Use Only. Not for use in diagnostic procedures.

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