Thermo Scientific™ GeneBLAzer™ PPAR delta DA Assay Kit

Product Code.	Quantity	unitSize
10073664	1 plate	Each

Product Code. 10073664 Supplier Thermo Scientific™ Supplier No. K1395

Description

GeneBLAzer™ PPAR delta DA (Division Arrested) cells and PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line.

GeneBLAzer™ PPAR delta DA (Division Arrested) cells and PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PPAR delta DA cells and PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of L-165041. In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
• Antagonists: 4-OH-tam+0.2 nM E2, Tam+0.2 nM E2, 4-OH-tamoxifen, Tamoxifen, RU486+0.2 nM E2, RU486
• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Division-Arrested Cells
• Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
  • PPAR delta HEK 293T DA cells (K1395A)
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Description: GeneBLAzer™ PPAR delta DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial at 4,800,000 cells/mL Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• Detection Method: Fluorescence
• For Use With (Application): Cellular imaging, pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Cell analysis, cell-based reporter assays, cell-based and lactamase assays, drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based ADME/tox assays, cellular adme assays, nuclear receptor cellular adme assays
• Gene ID (Entrez): 5467
• Gene Symbol: PPARδ
• Includes: PPAR delta HEK 293T DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg
• Primary Agonist: 17-β-estradiol
• Product Line: GeneBLAzer
• Quantity: 1 plate
• Reporter Gene: BLA (Beta-Lactamase)
• Sample Type: 293T (HEK)
• Target Entry: PPAR delta, NR1C2, PPARD
• Type: Division-Arrested Cells Assay Kit
• Cell Line: 293T (HEK)
• System Type: GeneBLAzer™
• Druggable Target: Nuclear Receptors

For Research Use Only. Not for use in diagnostic procedures.