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Thermo Scientific™ GeneBLAzer™ MC2R CHO-K1 DA Assay Kit
Description
The GeneBLAzer™ MC2R CHO-K1 DA (Division Arrested) cells contain the human melanocortin-2 receptor (MC2R), (Accession No. NM_000529.1) and the Melanocortin-2 receptor accessory protein (MRAP), (Accession No. NM_178817.3) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP Response Element (CRE).
The MC2R-CHOK1 DA cells are functionally validated for Z'-factor and EC50 concentrations of ACTH 1-24. In addition, MC2R-CRE-bla-CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
Specifications
| Accession Number | NM_000529.2 |
| Assay Entry | Cell-based beta lactamase reporter gene |
| Cell State | Division-Arrested Cells |
| Content And Storage | Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ MC2R CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ MC2R CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ MC2R CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
| Description | GeneBLAzer™ MC2R CHO-K1 DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage |
| Detection Method | Fluorescence |
| For Use With (Application) | Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays |
| Gene Symbol | MC2R |
| Includes | Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ MC2R CHO-K1 DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, GeneBLAzer™ MC2R CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis |
| Population Status | Single Cell Clone Origin |
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For Research Use Only. Not for use in diagnostic procedures.
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