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Thermo Scientific™ GeneBLAzer™ LXR beta DA Assay Kit

Product Code. 10115554
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Product Code. 10115554 Supplier Thermo Scientific™ Supplier No. K1415

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Description

GeneBLAzer™LXR beta DA (Division Arrested) cells and LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

GeneBLAzer™LXR beta DA (Division Arrested) cells and LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-LXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both LXR beta DA cells and LXR beta-UAS-bla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of TO901317. In addition, LXR beta-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists DHT
Antagonists Cyp Acetate, Spirolactone, OHF=
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
• LXR beta HEK 293T DA cells (K1415A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Description GeneBLAzer™ LXR beta DA DA Assay Kit, 1 Plate, ∽4, 800,000 No. of Cell, Liquid, Division-Arrested Cells State, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear Receptors Target, BLA (Beta-Lactamase) Reporter, Epithelial Cell Phenotype, Adherent Growth Properties
Detection Method Fluorescence
For Use With (Application) Cellular imaging, pharma and biopharma, target and lead identification and validation, target-based adme/tox assays
Gene ID (Entrez) 7376
Gene Symbol LXRβ
Includes LXR beta HEK 293T DA cells (K1415A) LiveBLAzer™-FRET B/G loading kit
Primary Agonist R1881
Product Line GeneBLAzer
Quantity 1 plate
Reporter Gene BLA (Beta-Lactamase)
Sample Type 293T (HEK)
Target Entry LXR beta, NR1H2, LXRB
Type Division-Arrested Cells Assay Kit
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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