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Thermo Scientific™ GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells

Product Code. 10145793
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Quantity:
2 x 106 Cells, 2 x 106 cells
Unit Size:
Each
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Product Code. 10145793

Brand: Thermo Scientific™ K1689B

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Description

ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line.

ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER beta-UAS-bla GripTite™293 cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Cell State Dividing Cells
Content And Storage ER beta-UAS-bla GriptTite™ cells (K1091)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen), Liquid nitrogen, room temperature
Detection Method Fluorescence, Fluorescent
Description GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells, 2 x 106 Cells, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Druggable Target Nuclear Receptors
Includes GeneBLAzer™ ER beta -UAS-bla GripTite™ cells Includes: ER beta-UAS-bla GripTite™ cells, Protocol, Certificate of Analysis
No. of Cells 2 x 106 Cells
Product Line GeneBLAzer
Quantity 2 x 106 Cells, 2 x 106 cells
Product Type GripTite Cells
Sample Type GripTite™ 293
Reporter Gene BLA (Beta-Lactamase)
Shipping Condition Room Temperature
Type Cell Line
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For Research Use Only. Not for use in diagnostic procedures.

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