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Thermo Scientific™ GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

Product Code. 10135793
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2 x 10^6 Cells, 2 x 106 cells
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10135793 2 x 10^6 Cells, 2 x 106 cells Each
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Product Code. 10135793 Supplier Thermo Scientific™ Supplier No. K1688

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Description

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line.

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line. GeneBLAzer™ UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™ cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Betamethasone, Cortisol, Budesonide
Antagonists Milfepristone, Cortisone, Progesterone
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage ER alpha-UAS-bla GriptTite™ cells (K1090)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen), Liquid nitrogen, room temperature
Detection Method Fluorescence, Fluorescent
Description GeneBLAzer™ ERα-UAS-bla GripTite™ Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 Cells/mL Quantity of Cells, Cortisone/Mifepristone/Progesterone Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Species Human
Druggable Target Nuclear Receptors
Includes ER alpha-UAS-bla GriptTite™ cells
No. of Cells 2 x 106 Cells
Primary Agonist Dexamethasone
Product Line GeneBLAzer
Quantity 2 x 10^6 Cells, 2 x 106 cells
Product Type GripTite Cells
Sample Type GripTite™ Cells
Reporter Gene BLA (Beta-Lactamase)
Shipping Condition Room Temperature
Target Entry ER alpha, ESR1, ERA, NR3A1
Type Cell Line
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For Research Use Only. Not for use in diagnostic procedures.

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