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Thermo Scientific™ GeneBLAzer™ EDNRA HEK 293T DA Assay Kit

Product Code. 10155173
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Product Code. 10155173 Supplier Thermo Scientific™ Supplier No. K1347

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Description

GeneBLAzer™ EDNRA HEK 293T DA (Division Arrested) cells and EDNRA-NFAT-bla HEK 293T cells contain the human Endothelin type A (EDNRA) receptor stably integrated into the CellSensor™ NFAT-bla HEK 293T cell line.

This cell line is available as dividing cells by requesting a quote for Cat. No. K1223 from dividingcells@invitrogen.com

GeneBLAzer™ EDNRA HEK 293T DA (Division Arrested) cells and EDNRA-NFAT-bla HEK 293T cells contain the human Endothelin type A (EDNRA) receptor stably integrated into the CellSensor™ NFAT-bla HEK 293T cell line. CellSensor™ NFAT-bla HEK 293T cells (Cat. No. K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (NFAT). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both EDNRA HEK 293T DA cells and EDNRA-NFAT-bla HEK 293T cells are functionally validated for Z'-factor and EC50 concentrations of ET-1 (Figure 1). In addition, EDNRA-NFAT-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Endothelin receptor type A (ENDRA) mediates signaling of endothelin proteins that regulate several critical biological processes, including development and function of blood vessels, production of certain hormones, and stimulation of cell growth and division. Defects in EDNRA-mediated signaling have been implicated in a wide range of diseases, including cancer, hypertension, and cardiovascular disorders.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001957
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage

GeneBLAzer™ EDNRA HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
• EDNRA HEK 293T DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D, 1 mL
• 2 protocols
• Certificate of Analysis

GeneBLAzer™ EDNRA HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
• EDNRA HEK 293T DA cells
• 1 protocol
• Certificate of Analysis

GeneBLAzer™ EDNRA-NFAT-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
• EDNRA-NFAT-bla HEK 293T cells
• 1 protocol
• Certificate of Analysis

Storage
GeneBLAzer™ EDNRA HEK 293T DA Cells and EDNRA-NFAT-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.
Description GeneBLAzer™ EDNRA HEK 293T DA Assay Kit, 1 Plate, ∽2,400,000 cells/vial Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Detection Method Fluorescence
For Use With (Application) Cellular imaging, pharma and biopharma, Cell analysis, cellular imaging, cell-based reporter assays, cell-based and beta-lactamase assays, drug discovery and development, target and lead identification and validation, g-protein coupled receptor biology, cell-based GPCR reporter assays, target-based ADME/tox assays, cellular adme assays, g-protein coupled receptor adme assays
Gene ID (Entrez) 1909
Gene Symbol EDNRA
Includes GeneBLAzer™ EDNRA HEK 293T DA Assay Kit Each system contains sufficient division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: EDNRA HEK 293T DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, 1mL, 2 protocols, Certificate of Analysis
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 1 plate
Reporter Gene BLA (Beta-Lactamase)
Sample Type 293T (HEK)
Target Entry EDNRA
Type Division-Arrested Cells Assay Kit
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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