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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Brand:  Invitrogen™ A21178

Code : NEW

Additional Details : Weight : 0.01000kg

Product Code. 15473903

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Alert:

For research use only. Not for use in diagnostic procedures.


Includes:
  • CRISPR OFP Nuclease Vector, linearized
  • 10X Oligonucleotide Annealing Buffer
  • DNase/RNase-free Water
  • 5X Ligation Buffer
  • T4 DNA Ligase
  • U6 Forward Sequencing Primer
  • Control, double stranded oligonucleotide
  • OneShot(TM) TOP10 Chemically Competent Cells

Description

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter.

GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells.

The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. Competent cells included for added convenience and highest cloning efficiencies.

GeneArt™ CRISPR Nuclease Vector system offers:
  • Easy-to-design genome engineering system
  • Affordable, ready-to-use cloning vectors
  • Enrichment for hard to transfect or difficult to modify cell
For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

Specifications

Restriction Enzyme/MCS
Primer-probe
Kit
CRISPR-Cas9
Crispr Nuclease Vector With ofP Reporter Kit
OFP fusion
10 Reactions
10 Reactions
Oligos (DNA)
• CRISPR OFP Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control, double stranded oligonucleotide
Store the above at -5 to -30°C.

• OneShot™ TOP10 Chemically Competent Cells
Store at -80°C.
Liquid
Fast
GeneArt™
U6, CMV
OFP
Gene Expression, Cloning
3' overhangs
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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.