Invitrogen™ GeneArt™ CRISPR Nuclease mRNA

Product Code. 15411054

Brand: Invitrogen™ A29378

Description

Invitrogen™ GeneArt™ CRISPR Nuclease mRNA is formulated using a new manufacturing process to achieve superior quality and improved product performance.

• This wild-type Cas9 mRNA has two nuclear localization signals (NLSs).
• Cas9 constructs with two NLSs have been shown to be more efficient at targeting the Cas9 protein to the nucleus (1).
• The mRNA format is ready to transfect and circumvents the need for time-consuming cloning steps required when using CRISPR vector systems.
• Cas9 mRNA is co-transfected with a target-specific guide RNA (gRNA) to direct the Cas9 protein to the intended genome locus to create a double-stranded break.
• The complete RNA format is a smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency.
• Additionally, the Cas9 mRNA can be used in multiplex approaches with more than one gRNA.
• Use this approach to determine which gRNA sequence works best for a particular target or to edit multiple genomic loci with one transfection.

Specifications

• Content And Storage: 1 vial CRISPR Nuclease mRNA. Store in ultra-cold freezer -68 to -85°C
• Quantity: 15 μg