GE Healthcare rmp Protein A Sepharose™ Fast Flow Affinity Media

Designed for high-purity separation of monoclonal and polyclonal antibodies

Brand: GE Healthcare

Manufacturer Part Number: 17-5138-02


UNSPSC: 41115700

Code: A0

Additional Details:
Additional Details: Weight: 0.07400kg

Product Code. 11349981

Quantity Price
1 £ 1854.62 / 25mL
Estimated Shipment
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Description and Specification


Quantity 25mL
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Requirements 2 to 8°C
Binding Capacity >Min. 22mg Human IgG/mL
Product Type rmp Protein A Sepharose fast flow media
For Use With (Application) For high-purity separation of monoclonal and polyclonal antibodies
Particle Size 60 to 165μm
Material Recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix
Matrix 4% Cross-Linked Agarose
pH Range 3 to 10
Storage Buffer 20% Ethanol
Ligand Type Recombinant Protein A (E. coli)

  • Non-mammalian-based media composed of recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix by reductive amination
  • Coupling chemistry that minimizes leakage of protein A ligand
  • Multi-point attachment of rProtein A by chemically-stable amide bonds
  • High capacity and good pressure/flow characteristics provide for high throughput

  • Matrix: 4% cross-linked agarose
  • Average particle size: 90μm (d50V)
  • Ligand: recombinant protein A (E. coli)
  • pH Stability working range: 3 to 10
  • pH Stability cleaning-in-place: 2 to 11
  • Binding capacity/mL chromatography medium: >35mg human IgG/mL
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column

For therapeutic applications requiring extremely pure eluate fractions of antibodies with non-exposure of antibodies to human or animal derivatives during purification process.