GE Healthcare Protein A and Protein G Mag Sepharose™


Overview
Brand: GE Healthcare

Manufacturer Part Number: 28-9513-78

X4 Protein A Mag Sepharose (4x500UL)

UNSPSC: 12161500

Code: A0

Additional Details:
Additional Details: Weight: 0.04000kg



Product Code. 10259574

Quantity Price
1 £ 403.13 / Pack of 4
Estimated Shipment
14-12-2016
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Description and Specification

Specification

Bead Type Protein A Mag Sepharose
Binding Capacity 8 to 17mg Human IgG/mL
For Use With (Application) Designed for covalent coupling of any Protein or biomolecule containing a free Amine group and is used for various types of pull down experiments, for biomarker research
Ligand Native Protein A
Matrix Paramagnetic spherical, highly cross linked agarose particles
Particle Size 37 to 100μm
Quantity 4 x 500μL
Storage Requirements 4° to 8°C

Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.

Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

  • Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C