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GE Healthcare IMAC Sepharose™ High Performance Media

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

Brand:  GE Healthcare 17-0920-07

Code : GE

Additional Details : Weight : 0.19400kg

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Product Code. 11534955

  • £871.02 / 100mL


Estimated Shipment
29-06-2018
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If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.

 

Description

Description

  • 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

Specifications

Specifications

<300cm/hr.
3 to 12
IMAC Sepharose HP
4° to 30°C
Purification of Polyhistidine tagged Proteins
34μm
100mL
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