GE Healthcare IMAC Sepharose™ High Performance Media

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

Brand: GE Healthcare

Manufacturer Part Number: 17-0920-07


UNSPSC: 41115700

Code: GE

Additional Details:
Additional Details: Weight: 0.19400kg

Product Code. 11534955

Quantity Price
1 £765.63 / 100mL
Estimated Shipment
Add to basket

If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.


Description and Specification


Flow Rate <300cm/hr.
For Use With (Application) Purification of Polyhistidine tagged Proteins
pH Range 3 to 12
Particle Size 34μm
Product Type IMAC Sepharose HP
Quantity 100mL
Storage Requirements 4° to 30°C

  • 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.