Cytiva TempliPhi™ Sequence Resolver Kit

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

Brand:  Cytiva 28-9035-31

Product Code. 10308259

Includes: Sample buffer, Reaction buffer and Enzyme mix

Description

• Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
• Requires 20 minutes hands-on time
• Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
• Improve difficult template sequencing success with up to 820 bp Phred 20 read length
• For use directly in cycle sequencing reactions without purification
• Available in sizes of 20, 50 and 500 reactions

Specifications

• Content And Storage: Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
• For Use With (Application): Amplification of difficult templates for successful DNA sequencing
• No. of Reactions: 200
• Test Time: 18 h

• Description: illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
• Includes: 1 x 900μL Sample Buffer, 1 x 900μL Reaction Buffer, 1 x 100μL Enzyme mix
• Product Type: illustra TempliPhi Sequence Resolver Kit

Safety and Handling

Product Identifier

• TempliPhi Sequence Resolver Kit, 200 reactions

Signal Word

• Warning

Hazard Category

• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P405-Store locked up.

Supplemental information

• MIXTURE LIST-Contains : glycerol