Cytiva Microspin G-50 Columns

Designed to allow the rapid purification of DNA for use in a wide range of applications using typical microfuge equipment. Cytiva illustra™ Microspin G-50 Columns will not remove or denature enzyme.

Brand:  Cytiva 27-5330-01

Product Code. 11753309

Description

• Optimal sample volumes in the range of 12-50μ
• For rapid purification of labeled DNA (>20 bases) from smaller molecules in solution
• Columns are prepacked with Sephadex G-50 Grade equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide
• illustra AutoSeq™ and illustra ProbeQuant™ G-50 Micro Columns are specifically designed for dye terminator removal and labelled probe purification respectively and offer optimized performance in those applications
• Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μL) following heat denaturation and phenol extraction
• Product recovery will be at least 10% lower than that from ProbeQuant G-50 Micro Columns.
• For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μl using spin-column chromatography

 

Desalting, buffer exchange; Removal of dye terminators from cycle sequencing reactions; Removal of labeled nucleotides from DNA labeling reactions

Order Info

Conveniently prepacked with Sephadex G-50 DNA Grade pre-equilibrated in TE buffer with 0.05% Kathon™ CG/ICP Biocide and collection tubes

Specifications

• For Use With (Application): Desalting, Buffer exchange, removal of dye terminators from cycle sequencing reactions, removal of labeled, Nucleotides in 25 to 50μL from DNA labeling reactions
• Includes: Sephadex™ G-50 DNA grade
• No. of Reactions: 50 Preps
• Product Type: Column