GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Brand: GE Healthcare

Manufacturer Part Number: 28-9537-66

UNSPSC: 41115711

Code: GE

Additional Details:
Additional Details: Weight: 0.10200kg

Product Code. 11618893

Quantity Price
1 £ 124.31 / Pack of 5
In Stock 1
Add to basket

Description and Specification


Flow Rate 1 to 4mL/min.
Diameter (Metric) Inner 0.7cm
Storage Requirements 4 to 8°C
Matrix 6% Cross-Linked Agarose
Material Polypropylene
Product Type HiTrap Talon™ crude prepacked Column
Particle Size 60 to 160μm
Ligand Type Talon™ Superflow Cobalt-IMAC
Packaging Quantity 5 x 1mL
Quantity 5 x 1mL
Binding Capacity Up to 20mg Histidine-tagged Protein1
Storage Buffer 20% Ethanol
pH Range 3 to 12
For Use With (Application) For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Max. Pressure 3 bar

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.