Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Brand:  Cytiva 28-9537-66

Product Code. 11618893

Description

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

• Prepacked with cobalt-based chromatography media
• Binding capacity approximately 7mg MBP
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
• 1 and 5mL columns

Media

• Ligand: TALON™ Superflow Cobalt-IMAC
• Matrix: 6% cross-linked agarose
• Average particle size: 60 to 160μm
• pH stability working range: 3 to 12

1mL columns

• Column I.D.: 7mm
• Flow velocity: 1 to 4mL/min.

5mL columns

• Column I.D.: 16
• Flow velocity: 5 to 20mL/min.

Specifications

• Quantity: 5 x 1 mL
• Storage Requirements: 4°C to 8°C
• Diameter (English) Inner: 0.27 in.
• Diameter (Metric) Inner: 0.7 cm
• Particle Size: 60 to 160 μm
• Max. Pressure: 3 bar
• pH Range: 3 to 12
• Ligand Type: Talon Superflow Cobalt-IMAC

• For Use With (Application): For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
• Binding Capacity: Up to 20 mg Histidine-tagged Protein1
• Product Type: HiTrap Talon crude prepacked Column
• Flow Rate: 1 to 4 mL/min
• Material: Polypropylene
• Matrix: 6% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol
• Packaging Quantity: 5 x 1 mL

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• EUH208-Contains cobalt. May produce an allergic reaction.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Ethanol, Methanol, Cobalt