GE Healthcare HiTrap™ rProtein A FF Columns

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. GE Healthcare Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17-5080-02

UNSPSC: 41106510

Code: A0

Additional Details:
Additional Details: Weight: 0.13800kg Transport: UN number : 1170 Chem class : 3 Pack group : III



Product Code. 11708548

Quantity Price
1 £ 2363.4 / Pack of 5
Estimated Shipment
21-03-2017
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Description and Specification

Specification

Storage Requirements 4° to 30°C
Flow Rate <20mL/min.
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Binding Capacity ≈50mg Human IgG/mL
Quantity 5 x 5mL
For Use With (Application) For preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, Serum and ascites

  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
  • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
  • Average particle size: 90μm
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Available as 1mL and 5mL columns
Media
  • Ligand: Recombinant protein A (E.coli)
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: ∽50mg human IgG/mL
  • pH stability working range: 3 to 10

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.