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GE Healthcare HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccarides, macromolecules with extended structures and small plasmids

Brand:  GE Healthcare 28-9356-04

Code : GX

Additional Details : Weight : 1.00000kg

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Product Code. 11330292

  • £500.15 / Each


Estimated Shipment
15-06-2018
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If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.

 

Description

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

Specifications

Specifications

Each
<1.3mL/min.
120mL
1.6cm
60cm
22psi
3 to 11
Polysaccharides, macromolecules with extended structures and even small plasmids
4° to 30°C
Low to medium pressure AKTA™ systems or pumped chromato graphy systems
HiPrep Sephacryl S-400 HR column
Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Polypropylene
25 to 75μm
Allyl Dextran and N, N'-methylenebisacrylamide
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