GE Healthcare HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
For reproducible protein purification, separating polysaccarides, macromolecules with extended structures and small plasmids
Brand: GE Healthcare 28-9356-06
Code : GX
Additional Details : CAS Number : 64-17-5 Weight : 1.00000kg
Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).
- Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
- Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
- Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
- Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)
- Average particle size: 50μm
- Matrix: Allyl dextran and N, N'-methylenebisacrylamide
- pH Stability working range: 3 to 11
- pH Stability cleaning range: 2 to 13
- Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
- Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
- Material (bed support): Polymethyl-Methacrylate
HiPrep 16/60 Sephacryl S-400 HR
- Fractionation [Mr] globular proteins: 2 x 104-8 x 106
- Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
- Fractionation range [Mr] DNA exclusion limit: 271bp
- Fractionation [Mr] Dextrans: 1 x 104-2 x 106
- Exclusion limit for Dextrans: 100 x 106
- Fractionation range [Mr] DNA exclusion limit: 1078bp
- Fractionation [Mr] Dextrans: 4 x 104-2 x 107
|3 to 11|
|Polysaccharides, macromolecules with extended structures and even small plasmids|
|4° to 30°C|
|Low to medium pressure AKTA™ systems or pumped chromato graphy systems|
|HiPrep Sephacryl S-500 HR column|
|Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids|
|25 to 75μm|
|Allyl Dextran and N, N'-methylenebisacrylamide|
Safety and Handling
- HiPrep 16/60 Sephacryl S-500 HR
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501-Dispose of contents/container to .
- MIXTURE LIST-Contains : ethanol