GE Healthcare HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccarides, macromolecules with extended structures and small plasmids

Overview
Brand: GE Healthcare

Manufacturer Part Number: 28935606

120 ML HIPREP 16/60 SEPHACRYL S-500 HR 120MLstore at ambient Store at Room Temperature

UNSPSC: 41104902

Code: GX

Additional Details:
Additional Details: CAS Number: 64-17-5 Weight: 1.00000kg



Product Code. 11350292

Quantity Price
1 £ 464.62 / Each
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Description and Specification

Specification

Quantity Each
Storage Requirements 4° to 30°C
Flow Rate <0.5mL/min.
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromato graphy systems
Bed Volume 120mL
Product Type HiPrep Sephacryl S-500 HR column
Diameter (Metric) Inner 1.6cm
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (Metric) 60cm
Material Polypropylene
Max. Pressure 22psi
Particle Size 25 to 75μm
pH Range 3 to 11
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107