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GE Healthcare™ Ficoll PM400

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. GE Healthcare™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

Brand:  GE Healthcare™ 17-0300-05

Code : GE

Additional Details : Weight : 6.60000kg

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Product Code. 11570724

  • £3724.00 / 5kg


Estimated Shipment
20-12-2017
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Please enquire with customer services on 01509 555500 for stock availability.

 

Description

Description

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
  • Has better osmotic properties than sucrose
  • Preserves functional and morphological integrity
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
  • Does not penetrate biological membranes
  • Serves as the raw material for preparation of Ficoll-Paque gradients
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
  • Supplied as dry powders

  • Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
  • Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
  • Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity

Specifications

Specifications

5kg
Produces density gradients to separate cells, subcellular components
≈17mL/g
Centrifugation Media
1.2g/mL
Powder
0.12EU/mg
4° to 30°C
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