GE Healthcare™ Ficoll PM400

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. GE Healthcare™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

Overview
Brand: GE Healthcare™

Manufacturer Part Number: 17-0300-50

FICOLL PM 400 500 G

UNSPSC: 12352100

Code: GE

Additional Details:
Additional Details: Weight: 0.61000kg



Product Code. 11590724

Quantity Price
1 £ 479.0 / 500g
Estimated Shipment
31-01-2017
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Description and Specification

Specification

Quantity 500g
Product Type Centrifugation Media
Shelf Life 5 years
Format Powder
Storage Requirements 4° to 30°C
Viscosity ≈17mL/g
Density 1.2g/mL
For Use With (Application) Produces density gradients to separate cells, subcellular components
Endotoxin Level 0.12EU/mg

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
  • Has better osmotic properties than sucrose
  • Preserves functional and morphological integrity
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
  • Does not penetrate biological membranes
  • Serves as the raw material for preparation of Ficoll-Paque gradients
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
  • Supplied as dry powders

  • Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
  • Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
  • Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity