GE Healthcare Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17-0440-01

CON-A SEPHAROSE 4B 100 ML

UNSPSC: 41115700

Code: GE

Additional Details:
Additional Details: Weight: 1.35620kg



Product Code. 11524905

Quantity Price
1 £ 531.42 / 100mL
Estimated Shipment
19-12-2016
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Description and Specification

Specification

Binding Capacity 30mg/mL
For Use With (Application) For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
Ligand Concanavalin A
Ligand Concentration 13mg Con A/mL Drained Medium
Matrix 4% Agarose
Particle Size 90μm
pH Stability 4 to 9
Quantity 100mL
Storage Requirements 2° to 8°C

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

  • Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars
  • Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
  • Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
  • Average particle size: 90μm
  • Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol