Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media

Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases

Brand:  Cytiva 17-0948-01

Product Code. 10271350

Description

This medium is well established as adsorbent for albumin and interferon at laboratory and process scale.

• Cibacron Blue 3G is covalently coupled to the base matrix allowing for low ligand leakage and high capacity
• The blue dye binds proteins, such as albumin, interferon, lipoproteins and blood coagulation factors
• The dye also binds enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+
• Avg. particle size: 90μm
• Volume: 50mL

Developed and tested in co-operation with large-scale manufacturers and is used in routine commercial production.

• For large-scale purification of interferon and albumin
• For isolating groups of enzymes and removing of albumin
• For industrial applications and other approved applications

Certifications

Certificate of Analysis

Specifications

• Quantity: 50 mL
• Stability: Stable in commonly used aqueous Buffers: 70% Ethanol, 6 M Guanidine Hydrochloric, 8 M Urea
• Product Type: Blue Sepharose 6 Fast Flow Media
• Particle Size: 45 to 165 μm
• Height (Metric) Bed: 25 cm
• Max. Pressure: 100 kPa
• pH Range: 4 to 12
• Ligand Type: Cibacron Blue

• For Use With (Application): For Albumin and Interferon at Both Laboratory and Process Scale
• Storage Requirements: 2°C to 8°C
• Flow Rate: 250 to 400 cm/Hoµr
• Height (English) Bed: 9.84 in.
• Includes: Certificate of Analysis
• Matrix: 6% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol, 0.1 M Potassium Phosphate Buffer

Safety and Handling

Product Identifier

• Blue Sepharose 6 Fast Flow, 50 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-1.2 per cent of the mixture consists of component(s) of unknown acute toxicity.
• UNKNOWN AQUATIC-Contains 16.5% of components with unknown hazards to the aquatic environment.