Eurofins DiscoverX GCK Protein, Active

Product Code.	Quantity	unitSize
17380644	10 μg	10µg
17390644	250 μg	250µg

Product Code. 17380644 Supplier Eurofins DiscoverX Supplier No. 14743

Description

Active, N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells, for use in Kinase Assays.

GCK (Glucokinase) is a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia.

GenBank BC047865

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Molecular Weight (g/mol): 80.6 kDa
• Quantity: 10 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal GST
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥38%
• Protein: GCK, active