Thermo Scientific™ Gaussia-Dura Luciferase Reporter Assay Vectors

Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 16190

X10 PMCS-GAUSSIA-DURA LUC VECTOR, DNA AT 0.5UG/µLin 10mM Tris-HCl, pH 8.5 10µg Store in Freezer at

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 0.22680kg



Product Code. 12381853

Quantity Price
1 £ 74.25 / 10µg
Estimated Shipment date
from Supplier 19-12-2016
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Description and Specification

Specification

Description pMCS-Gaussia-Dura Luc Vector
Formulation DNA at 0.5ug/uL in 10mM Tris-HCl, pH 8.5
Quantity 10µg
Sufficient For Gene cloning and Gaussia luciferase glow assays

The Gaussia Dura Luc Vectors contains a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization